Isolation and characterization of strains of rhizobium species in Pterogyne nitens Tull

Abstract

The legume Pterogyne nitens Tull. belonging to the subfamily Caesalpinoideae is a widely distributed species, hardiness and rapid growth, and these interesting characteristics for use in recovery of degraded areas. When in symbiosis with nitrogen fixing bacteria, these legumes are more tolerant to adverse conditions. Therefore, the objective of this work was isolate and to characterize strains of rhizobia in the specie Pterogyne nitens Tull. For this, Samples of soil from planting madeira nova were placed in 25 plastic pots of 500 mL. Then two mechanically scarified seeds were planted in each pot. After 120 days of the planting, the roots were analyzed for the presence of nodules and growth of rhizobia in culture medium 79. Moreover, were grown in culture medium, bacteria BR 3630, BR 6205, BR 6212 and BR 4406, acquired from Embrapa Agrobiologia, Seropédica - RJ, for comparison purposes. There were no nodules on the roots, but from extract of the roots was possible to obtain an isolate with characteristics similar strain Mesorhizobium elkanii (BR 3630) from Embrapa Agrobiology.