TOXICITY OF Rosmarinus officinalis ESSENCIAL OIL IN THE CONTROL OF Aspergillus brasiliensis FUNGUS

Authors

  • Lundoi Tobias Lee Programa de Pós-graduação em Tecnologia Ambiental, Escola de Engenharia Industrial Metalúrgica de Volta Redonda, Universidade Federal Fluminense (UFF) http://orcid.org/0000-0002-6055-3972
  • Sabrinna Aires Garcia Escola de Engenharia Industrial Metalúrgica de Volta Redonda, Universidade Federal Fluminense (UFF) http://orcid.org/0000-0003-4389-7420
  • Pedro Amorim Berbert LEAG Laboratório de Engenharia Agrícola UENF - Universidade Estadual do Norte Fluminense Darcy Ribeiro http://orcid.org/0000-0003-2480-8162
  • Carlos Eduardo de Souza Teodoro Programa de Pós-graduação em Tecnologia Ambiental, Escola de Engenharia Industrial Metalúrgica de Volta Redonda, Universidade Federal Fluminense (UFF) https://orcid.org/0000-0001-8591-0502
  • Ana Paula Martinazzo Programa de Pós-graduação em Tecnologia Ambiental, Escola de Engenharia Industrial Metalúrgica de Volta Redonda, Universidade Federal Fluminense (UFF) https://orcid.org/0000-0003-0704-2986

DOI:

https://doi.org/10.18316/rca.v17i1.8186

Keywords:

Rosemary, Alternative control, Natural products, Fungicidal properties, Fungal pathogens.

Abstract

Fungi are mainly responsible for spoiling food, resulting in losses, in addition to having the ability to produce toxins, which can be harmful to human and animal health. The control of these fungi occurs through the use of pesticides, which are harmful contaminants to the environment and health, in addition to the fact that these microorganisms become resistant to the action of these substances making them inefficient. Due to these factors, the search for alternative methods of controlling these microorganisms began. In this scenario, essential oils are shown as a possible alternative. The objective of this study was to identify the main components and to evaluate the effectiveness of rosemary essential oil (Rosmarinus officinalis) for controlling the fungus Aspergillus brasiliensis. The main components of essential oil, identified by gas chromatography, in order of concentration, were: 1.8-cineole (48%), camphor (12%), and β-pinene (8%). The effect of essential oils on the mycelial growth of the fungus was verified in vitro tests between doses of 0.8 to 25 µL / mL, this being the dose with the greatest fungal control. Based on the results of the in vitro tests, serial microdilution was performed in microplates to find the minimum inhibitory concentration, which was 25 µL / mL. The results showed that the essential oil showed fungicidal potential on A. brasiliensis.

References

ADAMS, R. P. 2007. Identification of essential oil components by gas chromatography/mass spectrometry (4th editio). Carol Stream. 804p.

ALVES, V. C., CARDOSO FILHO, F. C., PEREIRA, M. M. G., COSTA, A. P. R., & MURATORI, M. C. S. 2014. Identificação de espécies de Aspergillus e Potencial Toxígeno de Aspergillus flavus isolados de rações comerciais. Revista Científica de Produção Animal, 16(2), 131–136. https://doi.org/10.15528/2176-4158/rcpa.v16n2p131-136

ARAÚJO, S. G., PINTO, M. E. A., SILVA, N. L., SANTOS, F. J. L. DOS, CASTRO, A. H. F., & LIMA, L. A. R. D. S. 2013. Atividades antioxidante e alelopática do extrato e frações obtidos de Rosmarinus officinalis. BBR - Biochemistry and Biotechnology Reports, 2(1), 35–43. https://doi.org/10.5433/2316-5200.2013v2n1p35

BAGHLOUL, F., MANSORI, R., & DJAHOUDI, A. 2017. In vitro antifungal effect of Rosmarinus officinalis essential oil on Aspergillus niger. National Journal of Physiology, Pharmacy and Pharmacology, 7(3), 1. https://doi.org/10.5455/njppp.2017.7.7021513102016

BARATTA, M. T., DORMAN, H. J. D., DEANS, S. G., FIGUEIREDO, A.C., BARROSO, J. G., & RUBERTO, G. 1998. Antimicrobial and antioxidant properties of some commercial essential oils. Flavour and Fragrance Journal, 13, 235–244. https://doi.org/10.1002/(SICI)1099-1026(1998070)13:4<235::AID-FFJ733>3.0.CO;2-T

BEDINI, S., GUARINO, S., ECHEVERRIA, M. C., FLAMINI, G., ASCRIZZI, R., LONI, A., & CONTI, B. 2020. Allium sativum, Rosmarinus officinalis, and Salvia officinalis essential oils: A spiced shield against blowflies. Insects, 11(3). https://doi.org/10.3390/insects11030143

CAMILETTI, B. X., ASENSIO, C. M., DE LA PECCI, M. DE LA P. G., & LUCINI, E. I. 2014. Natural control of corn postharvest fungi Aspergillus flavus and Penicillium sp. using essential oils from plants grown in Argentina. Journal of Food Science, 79(12), M2499–M2506. https://doi.org/10.1111/1750-3841.12700

CHAO, S. C., YOUNG, D. G., & OBERG, C. J. 2000. Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. Journal of Essential Oil Research, 12(5), 639–649. https://doi.org/10.1080/10412905.2000.9712177

CHOI, H. S. 2003. Character impact odorants of Citrus Hallabong [(C. unshiu Marcov x C. sinensis Osbeck) x C. reticulata Blanco] cold-pressed peel oil. Journal of Agricultural and Food Chemistry, 51(9), 2687–2692. https://doi.org/10.1080/10412905.2000.9712177

da SILVA BOMFIM, N., KOHIYAMA, C. Y., NAKASUGI, L. P., NERILO, S. B., MOSSINI, S. A. G., ROMOLI, J. C. Z., GRATON MIKCHA, J. M., ABREU FILHO, B. A. DE, & MACHINSKI, M. 2020. Antifungal and antiaflatoxigenic activity of rosemary essential oil (Rosmarinus officinalis L.) against Aspergillus flavus. Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, 37(1), 153–161. https://doi.org/10.1080/19440049.2019.1678771

de BILLERBECK, V. G., ROQUES, C. G., BESSIÈRE, J. M., FONVIEILLE, J. L., & DARGENT, R. 2001. Effects of Cymbopogon nardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger. Canadian Journal of Microbiology, 47(1), 9–17. https://doi.org/10.1139/cjm-47-1-9

DELLAVALLE, P. D., CABRERA, A., ALEM, D., LARRAÑAGA, P., FERREIRA, F., & RIZZA, M. D. 2011. Antifungal activity of medicinal plants extracts against phytopathogenic fungus Alternaria spp. Chilean Journal of Agricultural Research, 71(June), 231–239. https://doi.org/10.1007/s11418-007-0216-x

DESAI, M. A., PARIKH, J., & DE, A. K. 2014. Modelling and optimization studies on extraction of lemongrass oil from Cymbopogon flexuosus (Steud.) Wats. Chemical Engineering Research and Design, 92(5), 793–803. https://doi.org/10.1016/j.cherd.2013.08.011

FERDES, M., AL JUHAIMI, F., ÖZCAN, M. M., & GHAFOOR, K. 2017. Inhibitory effect of some plant essential oils on growth of Aspergillus niger, Aspergillus oryzae, Mucor pusillus and Fusarium oxysporum. South African Journal of Botany, 113, 457–460. https://doi.org/10.1016/j.cherd.2013.08.011

FERREIRA, D. F. 2014. Sisvar: A Guide for its Bootstrap procedures in multiple comparisons. Ciência e Agrotecnologia, 38(2), 109–112. https://doi.org/10.1590/S1413-70542014000200001

GUIMARÃES, L. G. D. L., CARDOSO, M. D. G., DE SOUSA, P. E., DE ANDRADE, J., & VIEIRA, S. S. 2011. Atividades antioxidante e fungitóxica do óleo essencial de capim-limão e do citral. Revista Ciência Agronômica, 42(2), 464–472. https://doi.org/10.1590/S1806-66902011000200028

HENDEL, N., NAPOLI, E., SARRI, M., SAIJA, A., CRISTANI, M., NOSTRO, A., GINESTRA, G., & RUBERTO, G. 2019. Essential oil from aerial parts of wild algerian rosemary: Screening of chemical composition, antimicrobial and antioxidant activities. Journal of Essential Oil Bearing Plants, 22(1), 1–17. https://doi.org/10.1080/0972060X.2019.1590246

HÖGNADÓTTIR, Á., & ROUSEFF, R. L. 2003. Identification of aroma active compounds in orange essence oil using gas chromatography-olfactometry and gas chromatography-mass spectrometry. Journal of Chromatography A, 998(1–2), 201–211. https://doi.org/10.1016/S0021-9673(03)00524-7

KASPER, A. A. M., SOUSA, S. F. DE, SOUSA JÚNIOR, J. J. V. DE, ESCHER, S. K. S., & BARATA, L. E. S. 2018. Comparação da atividade antifúngica do óleo comercial e do extrato etanólico das folhas de nim (Azadirachta indica Juss) frente a fungos fitopatogênicos. Revista Ibero-Americana de Ciências Ambientais, 9(6), 54–62. https://doi.org/10.6008/cbpc2179-6858.2018.006.0007

KNAAK, N., & FIUZA, L. 2010. Potencial dos óleos essenciais de plantas no controle de insetos e microrganismos. Neotropical Biology and Conservation, 5(2), 120–132. https://doi.org/10.4013/nbc.2010.52.08

LANÇAS, F. M. 2009. A cromatografia líquida moderna e a espectrometria de massas: Finalmente “compatíveis”?. Scientia Chromatographica, 5(1), 27–46. https://doi.org/10.4322/sc.2013.005

LORENZI, H. (2008). Plantas medicinais no Brasil: nativas e exo?ticas (Instituto Plantarum (ed.); 2 nd). Instituto Plantarum de Estudos da Flora. 297p. https://books.google.com.br/books?id=xPEfAQAAIAAJ&q=Plantas+medicinais+no+Brasil:+nativas+e+exóticas.&dq=Plantas+medicinais+no+Brasil:+nativas+e+exóticas.&hl=pt-BR&sa=X&ved=0ahUKEwiC3qzZu8DcAhVMhpAKHVkmDZwQ6AEIJzAA

MEKONNEN, A., YITAYEW, B., TESEMA, A., & TADDESE, S. 2016. In vitro antimicrobial activity of essential oil of Thymus schimperi, Matricaria chamomilla, Eucalyptus globulus, and Rosmarinus officinalis. International Journal of Microbiology, 2016. https://doi.org/10.1155/2016/9545693

OLIVEIRA, R. C., CARVAJAL-MORENO, M., MERCADO-RUARO, P., ROJO-CALLEJAS, F., & CORREA, B. 2020. Essential oils trigger an antifungal and anti-aflatoxigenic effect on Aspergillus flavus via the induction of apoptosis-like cell death and gene regulation. Food Control, 110, 107038. https://doi.org/10.1016/j.foodcont.2019.107038

PANDEY, A. K., RAI, M. K., & ACHARYA, D. 2003. Chemical composition and antimycotic activity of the essential oils of corn mint (Mentha arvensis) and Lemon Grass (Cymbopogon flexuosus) against human pathogenic fungi. Pharmaceutical Biology, 41(6), 421–425. https://doi.org/10.1076/phbi.41.6.421.17825

PARÌŒENICOVAÌ, L., SKOUBOE, P., FRISVAD, J., SAMSON, R. A., ROSSEN, L., TEN HOOR-SUYKERBUYK, M., & VISSER, J. 2001. Combined molecular and biochemical approach identifies Aspergillus japonicus and Aspergillus aculeatus as two species. Applied and Environmental Microbiology, 67(2), 521–527. https://doi.org/10.1128/AEM.67.2.521-527.2001

SATYAL, P., JONES, T., LOPEZ, E., MCFEETERS, R., ALI, N., MANSI, I., AL-KAF, A., & SETZER, W. 2017. Chemotypic characterization and biological activity of Rosmarinus officinalis. Foods, 6(3), 20. https://doi.org/10.3390/foods6030020

TATSADJIEU, N. L., YAOUBA, A., NUKENINE, E. N., NGASSOUM, M. B., & MBOFUNG, C. M. F. 2010. Comparative study of the simultaneous action of three essential oils on Aspergillus flavus and Sitophilus zeamais Motsch. Food Control, 21(2), 186–190. https://doi.org/10.1016/j.foodcont.2009.05.004

VARDAR-ÜNLÜ, G., CANDAN, F., SÓKMEN, A., DAFERERA, D., POLISSIOU, M., SÖKMEN, M., DÖNMEZ, E., & TEPE, B. 2003. Antimicrobial and antioxidant activity of the essential oil and methanol extracts of Thymus pectinatus Fisch. et Mey. Var. pectinatus (Lamiaceae). Journal of Agricultural and Food Chemistry, 51(1), 63–67. https://doi.org/10.1021/jf025753e

VARGA, J., KOCSUBE, S., TOTH, B., FRISVAD, J. C., PERRONE, G., SUSCA, A., MEIJER, M., & SAMSON, R. A. 2007. Aspergillus brasiliensis sp. nov., a biseriate black Aspergillus species with world-wide distribution. International Journal of Systematic and Evolutionary Microbiology, 57(8), 1925–1932. https://doi.org/10.1099/ijs.0.65021-0

YU, J.-H. 2010. Regulation of Development in Aspergillus nidulans and Aspergillus fumigatus. Mycobiology, 38(4), 229-237. https://doi.org/10.4489/myco.2010.38.4.229

ZHANG, H., CHEN, F., WANG, X., & YAO, H. Y. 2006. Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Research International, 39(8), 833-839. https://doi.org/10.1016/j.foodres.2006.03.007

Downloads

Published

2023-07-28

Issue

Vol. 17 No. 1 (2023)

Section

Artigos

License

Authors must submit their manuscripts to be published in this journal agree with the following terms:

  1. Authors maintain the copy rights and concede to the journal the right of first publication, with the paper simultaneously licensed under the License Creative Commons attribution that permits the sharing of the paper with recognition of authorship and initial publication in this journal.

  2. Since the articles are presented in this journal of public access, they are of free use, with their own attributions for educational and non-commercial purposes.

 

Licença Creative Commons

The RCA Journal - REVISTA DE CIÊNCIAS AMBIENTAIS in: http://www.revistas.unilasalle.edu.br/index.php/Rbca was licensed with a Creative Commons License Creative Commons - Attribution - Noncommercial 3.0 Not Adapted.